apolipoprotein j apoj  (R&D Systems)

Journal: Nature Communications

Article Title: Podocyte-specific KLF6 primes proximal tubule CaMK1D signaling to attenuate diabetic kidney disease

doi: 10.1038/s41467-024-52306-5

Figure Lengend Snippet: a , b OCR and quantifications in 1° PT treated with conditioned media (CM) from 1° KLF6 PODTA (OE) versus NPHS2-rtTA (WT) podocytes in normal glucose (NG) and high glucose (HG). n (biological replicates) = 7; basal respiration: p = 0.0013:OE CM (NG) vs WT CM (HG), p = 0.0008:OE CM (NG vs HG), p = 0.0002:WT CM vs OE CM (HG); ATP production: p = 0.0006:OE CM (NG) vs WT CM (HG), p = 0.0004:WT CM (NG vs HG), p = < 0.0001:WT CM vs OE CM (HG); maximal respiration: p = 0.0343:WT CM (NG vs HG), p = 0.0389:OE CM (NG) vs WT CM (HG); spare respiratory capacity: p = 0.0143:OE CM (NG) vs WT CM (HG), p = 0.0117:WT CM (NG vs HG). c , d Volcano plot showing podocyte secretome and urine proteome from mice ( n = 3). e Circos plot highlighting ligand-receptor interactions for upregulated proteins in podocyte secretome and DEGs in preconditioned-PT from snRNA-seq. f Representative images of ApoJ immunohistochemistry. Arrows-ApoJ staining ( n = 3 mice/group, scale bar = 25 µm). g , h OCR and quantifications in 1° PT cells treated with CM + anti-ApoJ blocking antibody or CM + anti-IgG in HG ( n (biological replicates) = 7); WT CM vs OE CM: p = 0.0259(basal respiration), p = 0.0026(maximal respiration), p = 0.0004(spare respiratory capacity). i , j OCR and quantifications in 1° PT treated with CM from differentiated human podocytes in HG ( n (biological replicates) = 6, p = 0.0001(basal respiration), p = 0.0009(ATP production), p = 0.005(maximal respiration), p = 0.0156(spare respiratory capacity)). k Western blot for immunoprecipitation of ApoJ in 1° PT treated with CM in NG/HG. l , m Representative images from co-immunostaining for ApoJ, CaMK1D, Lrp2, and Hoechst. Arrows-indicate ApoJ/CamK1D and ApoJ/Lrp2 colocalization. (scale bar = 50 µm, 5 µm for inset). n , o OCR and quantification in 1° PT treated with recombinant ApoJ ± cilastatin/VEH ( n (biological replicates) = 7 for control), ( n (biological replicates)=5) for ApoJ+VEH, (n(biological replicates) = 5) for ± cilastatin; basal respiration: p = 0.0003(control vs ApoJ + VEH), p = 0.0157(control vs cilastatin), p = 0.0329 (ApoJ + VEH vs ApoJ + cilastatin); ATP production: p = 0.0001 (control vs ApoJ + VEH), p = 0.0086 (ApoJ + VEH vs ApoJ + cilastatin); maximal respiration: p = 0.0151 (control vs ApoJ + VEH). p Proposed schematic of potential KLF6-ApoJ-CaMK1D signaling between podocytes and PT cells. For all data: * p < 0.05, ** p < 0.01 *** p < 0.001, Kruskal–Wallis test with Dunn’s posttest; data presented as mean ± SEM. Source data are provided as a Source Data file. p Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en .

Article Snippet: Mouse 1° PT cells were treated with recombinant mouse ApoJ-His-tag (His-ApoJ) (20 µg/ml) (SinoBiological, 50485-M08H) and Cilastatin (0.1 mg/ml) (MedChemExpress, HY-A0166A), for 24 h under NG conditions.

Techniques: Immunohistochemistry, Staining, Blocking Assay, Western Blot, Immunoprecipitation, Immunostaining, Recombinant, Control

Journal: Molecular metabolism

Article Title: LRP1 in GABAergic neurons is a key link between obesity and memory function.

doi: 10.1016/j.molmet.2024.101941

Figure Lengend Snippet: Figure 1: Selective deletion of LRP1 in GABAergic neurons causes metabolic disorders. (A) Body weight, (B) epididymal fat, (C) serum leptin, (D) serum FFA, (E) serum ApoJ, (F) blood glucose, (G) serum insulin, and (H) HOMA-IR were measured in LRP1loxP/loxP and Vgat-Cre; LRP1loxP/loxP male mice that were overnight fasted mice at 32 weeks of age. n ¼ 12 for control, n ¼ 18 for Vgat-Cre; LRP1loxP/loxP. (I) Body weight, (J) blood glucose, (K) serum insulin, (L) serum leptin, and (M) serum ApoJ were measured in LRP1loxP/loxP and Vgat-Cre; LRP1loxP/loxP male mice that were overnight fasted at 16 weeks of age. n ¼ 12 for control, n ¼ 7 for Vgat-Cre; LRP1loxP/loxP. All graphs represent means or individual values SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. LRP1loxP/loxP by two-sided Student’s t-test. Q5

Article Snippet: Serum levels of insulin (90080, Crystal Chem, Chicago, IL), leptin (90030, Crystal Chem, Chicago, IL), apolipoprotein J (ApoJ) (MCLU00, R&D Systems, Minneapolis, MN), Ab40 (KMB3481, Thermo Fisher Scientific Inc., Waltham, MA), Ab42 (KMB3441, Thermo Fisher Scientific Inc., Waltham, MA) and free fatty acids (FFA) (ab65341, Abcam, Waltham, MA) were measured by enzyme-linked immunosorbent assay (ELISA).

Techniques: Control

Journal: Molecular metabolism

Article Title: LRP1 in GABAergic neurons is a key link between obesity and memory function.

doi: 10.1016/j.molmet.2024.101941

Figure Lengend Snippet: Figure 4: Relationship of motor function, motor coordination, and spatial recognition memory with obesity-linked metabolic parameters. Correlation of body weight with (A) total distance traveled on the locomotion test, (B) latency to fall on the rotarod test, (C) total distance traveled, and (D) time spent in the novel arm on the spatial Y-maze test was evaluated. Correlation of serum insulin with (E) latency to fall on the rotarod test, (F) total distance traveled, and (G) time spent in the novel arm on the spatial Y-maze test was evaluated. Correlation of serum leptin with (H) latency to fall on the rotarod test, (I) total distance traveled, and (J) time spent in the novel arm on the spatial Y-maze test was evaluated. Correlation of serum ApoJ with (K) latency to fall on the rotarod test and (L) time spent in the novel arm on the spatial Y-maze test were evaluated. The P values were obtained by Pearson correlation analysis, and the r values indicate Pearson correlation coefficient. All correlations were analyzed from the data of LRP1loxP/loxP and Vgat-Cre; LRP1loxP/loxP male mice (32 weeks old).

Article Snippet: Serum levels of insulin (90080, Crystal Chem, Chicago, IL), leptin (90030, Crystal Chem, Chicago, IL), apolipoprotein J (ApoJ) (MCLU00, R&D Systems, Minneapolis, MN), Ab40 (KMB3481, Thermo Fisher Scientific Inc., Waltham, MA), Ab42 (KMB3441, Thermo Fisher Scientific Inc., Waltham, MA) and free fatty acids (FFA) (ab65341, Abcam, Waltham, MA) were measured by enzyme-linked immunosorbent assay (ELISA).

Techniques:

Journal: Molecular metabolism

Article Title: LRP1 in GABAergic neurons is a key link between obesity and memory function.

doi: 10.1016/j.molmet.2024.101941

Figure Lengend Snippet: Figure 5: Relationship between metabolic parameters and cognitive function. Correlation of body weight, serum leptin, serum insulin, and serum ApoJ with the data from acquisition (A, C, E, G) and reversal (B, D, F, H) water T-maze on day 1 was evaluated. The P values were obtained by Pearson correlation analysis and r values indicate the Pearson correlation coefficient. All correlations were analyzed from the data of LRP1loxP/loxP and Vgat-Cre; LRP1loxP/loxP male mice (32 weeks old).

Article Snippet: Serum levels of insulin (90080, Crystal Chem, Chicago, IL), leptin (90030, Crystal Chem, Chicago, IL), apolipoprotein J (ApoJ) (MCLU00, R&D Systems, Minneapolis, MN), Ab40 (KMB3481, Thermo Fisher Scientific Inc., Waltham, MA), Ab42 (KMB3441, Thermo Fisher Scientific Inc., Waltham, MA) and free fatty acids (FFA) (ab65341, Abcam, Waltham, MA) were measured by enzyme-linked immunosorbent assay (ELISA).

Techniques: